Real time RT PCR was performed using BTV SEG1 RSA and BTV SEG1 UNI primers and BTV SEG1 probe (Table1). Real time RT PCR and conventional RT PCR were performed as described by Shaw and others (2007).View this table:View inlineView popupTABLE1: Primer and probe sequences used for RT PCR, sequencing and real time RT PCR assays for the detection of BTVConventional RT PCR was used for the sequence analysis of positive samples obtained via real time RT PCR using ORBI UNI F and ORBI UNI R sequence primers and a Qiagen OneStep RT PCR Kit (catalogue number: 210212, Qiagen, Germany), which amplifies a 412bp section of the orbivirus segment 1 gene section, as described by Shaw and others (2007).Sequence analysisThe sequencing analysis was performed using purified PCR products. The nucleotide sequence, TR AFYON2012 wholesale jerseys, was published with the accession number Tamura and others 2011).
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