To determine the characteristics of Piezo1 channels overexpressed in HEK293 cells or endogenously expressed in endothelial cells, whole cell and single channel patch clamp recordings will be used. Piezo1 plasmids will be transfected into the HEK 293 cells with lipofectamine transfection reagent. Endothelial cells will be freshly isolated from mouse second order mesenteric arteries.
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